Study Guide to Krikorian & Berquam

What was Haberlandt’s stated purpose? On what theory was this based?

How was Haberlandt unfortunate in his choice of Explants? Consider all aspects of this question.

Why was the use of photosynthetic cells a good idea and a bad idea considering his stated purpose?

Why was the choice of Epidermal Hairs a good idea and a bad idea considering his stated purpose?

What was good concerning the culture media that Haberlandt used in terms of what essential ingredients are required for plant cell culture.

What suggestions did he make for further development of better culture media and were his ideas validated by later workers?

What other ideas did he have concerning factors that might promote in vitro growth of plant cells?

What do Krikorian and Berquam regard as Haberlandt’s greatest theoretical statement regarding plant cell culture? The word begins with a T!

How did his work on wounding contribute to plant cell & tissue culture?

What is truly remarkable about this article by Haberlandt despite the fact that he never observed one cell Division?

PS: The only tantalizing observation that Haberlandt made was the migration of the nucleus to a central position in the cell. We now know that this often represents the first step in the mitosis of parenchyma cells. He had no way to know this.