BOT 612 Study Guide for Haberlandt’s Paper

When Did Haberlandt do most of his Experiments?

How does this compare with the formalization of the Cell Theory?

Why did he believe that single cells could divide and regenerate?

Why did he limit the choice of starting material?

Why did he culture Photosynthetic Cells?

What environmental factors did he test in one way or another?

Did he consider using sterile (axenic) culture conditions and did he take any precautions?

What is a hanging drop culture?

What type of culture medium components did he test? Don’t list all of the ingredients but group them appropriately.

What is one glaring criticism of the way in which he conducted his experiments?

What kinds of changes did he note in the cytology of cultured cells? Don’t list each and every thing but note the kinds of features he documented.

Be sure to Google up all of the plants that he used and note whether they are monocots or dicots.

What is the Engelmann Technique?

Did he note evidence of Growth in his experiments? If so what kinds of growth did he document?

Did he culture cells that were undifferentiated (meristem cells are undifferentiated) or highly differentiated cells?

The term Totipotent did not exist in his time. A Totipotent cell is one that can form an entire organism preferably via embryogenesis. Did Haberlandt think that this was possible?

What suggestions did Haberlandt make for future investigations of single cell cultures?

How many of these were ultimately shown to be important for cell and tissue culture establishment, and the regeneration of plants from them?