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Internal Leaf Anatomy

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Leaf Anatomy - General Features of the Lamina (Blade)

The Three Tissue Systems are Omnipresent!  

Epidermal - Ground (Mesophyll) - Vascular (Veins)

Textbook Leaves

TypDicotLeaf500Lab.jpg (47885 bytes)
Typical Dicot Leaf

SCaneVBundsX600Lab.jpg (182846 bytes)   TypMonoLeafLab.jpg (92462 bytes)
Cross Sections of Sugar Cane Leaf a Textbook Monocot

Leaf Anatomy - Lamina or Blade

The Adaxial Epidermis generally has a thick cuticle on the upper (adaxial) surface. Secretory & Non-secretory Trichomes may be present.

Stomata may occur on the adaxial epidermis but these are most frequent on the lower (abaxial) surface.

The Abaxial Epidermis usually has a thin cuticle. However, it may have a dense layer of trichomes which increase the "boundary layer" effect. This decreases the rate of transpiration.

Stomata are routinely present on the abaxial surface and are usually more abundant than on the abaxial surface when both surfaces have stomata.

OhiaPubPol.jpg (47636 bytes)

Lamina from ohia' lehua Metrosideros polymorpha showing a thick adaxial cuticle (C) and abaxial Trichomes (T)

Same but with Polarizing Optics. This brings out trichomes, crystals and cells with thick, highly organized Cell Walls

Mesophyll

Palisade mesophyll is composed of columnar cells that can be one to several layers thick. It is most common on the adaxial side of the leaf where sunlight is usually most abundant. Palisade parenchyma are more efficient for photosynthesis and water retention because of their large surface areas but small intercellular air spaces.

PalisadSEM240.jpg (14292 bytes)
SEM of Palisade Parenchyma

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Palisade in a Sun Leaf

Spongy mesophyll SpongyLab.jpg (20605 bytes)is usually found on the abaxial side of the leaf. It may consist of stellate parenchyma or chains of elongate cells which have large, lateral intercellular spaces. This allows rapid diffusion of gases like CO2.

SpongySEM240.jpg (24463 bytes)
SEM of Spongy Mesophyll

Note the large air spaces! The cells have a stellate appearance and resemble the jacks used by children.

Some plants have a uniform mesophyll which is composed of relatively isodiametric cells.

TiLfTBLModMod500.jpg (119324 bytes)
ti Lamina X-sectiom

WheatMesoMM300.jpg (25982 bytes)
Mesophyll Cells in the Wheat Lamina do not have pronounced shapes, and give a uniform appearance.

Nerium oleander has pronounced Palisade and Spongy Layers

NeriumLMPalisSpong300.jpg (40055 bytes)

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