| General Viewing | |
| Focus on specimen with Low Power Objective (2.5X, 4X or 6.3X). | |
| Focus Oculars (especially the one with the photo reticule). | |
| Set "Optivar" to 1.25. | |
| Swing up High Power Condenser Lens | |
| Change Objective to 10X or 16X. | |
| Focus on Specimen. | |
| Move Specimen out of the field of view. | |
| Make sure that Condenser Iris is Completely OPEN. | |
| Close Field Diaphragm COMPLETELY. | |
| Focus the Condenser so that Field Diaphragm is as SMALL as possible. | |
| Center the Condenser. | |
| Expand the Field Diaphragm to the edge of the Field of View. | |
| Fine-Center the Condenser. | |
| Expand the Field Diaphragm JUST BEYOND the Field of View. | |
| Adjust the Condenser Iris (16X and above). | |
Photomicroscopy |
|
| Check to see that the Correct
Filters are being used. The built-in Blue Daylight Filter must always be used. Place a second Bluefilter over the Field Diaphragm for unstained and Phloroglucinol-stained material. Remove the second Blue Filter for Toluidine Blue-Stained samples. |
|
| Select a 10 - 40X Objective & Focus on Specimen. | |
| Select the General Area for Photography. | |
| Pull out the Beam Path Control Knob to the Black Line. | |
| Select the Specific Area to be photographed. | |
| Align Specimen by Rotating the Stage. | |
| Adjust the final Magnification with "Optivar". | |
| Move the Specimen Out of the Field of View | |
| Focus the Ocular for the Photo-Reticule. | |
| Focus, Center & Expand the Field Diaphragm | |
| Move the Specimen back into the Field & Increase Illumination for Fine Focusing | |
| Adjust the Condenser Iris (especially for 25X & above) | |
| Reduce Illumination to the Blue Line & Press the A Button | |
TOP