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Sliding Microtome has three main parts. These are a] Knife Holder b] Slideway c] Specimen Holder WARNING - Microtome Knives are EXTREMELY SHARP & Dangerous! They are so sharp that you can hardly feel them cutting into your hand (DTW personal experience)! Be constantly aware of the blade's location! Allways use a handle to carry a Microtome Knife! Samples are secured in a Specimen Holder. There is a knob which is used to clamp the specimen in place. If the specimen is delicate, it needs to be encased in a support material. The height of the specimen is regulated by a lower knob on the specimen holder. There is a lever which locks the specimen holder in place. The Specimen Holder needs to be completely withdrawn at the start. The Specimen needs to protrude above the top of the Specimen Holder by 5-10 mm. Its height depends on its size and strength.
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