Stain-Phloroglucinol 
Directory
Stain-Phloroglucinol
Directory
Phloroglucinol-HCl - This stains lignified and suberized cell walls red-orange. The stain is colorless until it reacts with lignin or suberin. The reaction may take several minutes and works best without a coverslip. If you are going to use several stains or look at several specimens, stain with Phloroglucinol first, and set these aside until you have finished other things. a] Place several sections in Phloroglucinol on a microscope slide. b] Wait a few minutes c] Add a coverslip & observe You can leave the phloroglucinol in place or rinse out with water, as described above & below. This stain contains 20% HCl. Consequently, clean up any spills, especially on the microscope stage, and avoid getting this on yourself. |
 Gritty "stone cells" from pear flesh. The red stain indicates the presence of lignin.  An individual Cell from one of the clusters above.  A cluster of Lignified Fibers stained with Phloroglucinol |