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| A carrot root makes a good support for hand sections. Trim a carrot to a size that is easy to hold and is large enough to hold the specimen. Blot the outer cut surfaces so that they are not slipery. Make a slit or opening in the carrot that will accomadate the specimen. Place the specimen into the slit. Squeeze the carrot with your fingers to secure the specimen. Make a transverse slice with a razor blade to provide a uniform flat surface. Slice sections rapidly as described earlier & float them in a Petri dish of water. Treat these like free-hand sections. If carrots are unavailable, find a local plant that has similar properties and use it. Use of a carrot will result in cellular debris which may contaminate the sections. This may be a small price to pay with difficult specimens. Look for this in the carrot microphotos. |