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are a couple of ways to make hand sectioning safer and easier. These involve the use of a support substance to hold the specimen
and make it easier to get thin sections. The tried & true way to do this is to use commercial Pith. Soak the Pith in water. Make a slit or opening in the Pith that will accomadate the specimen. Place the specimen into the Pith. Squeeze the Pith with your fingers to secure the specimen. Make a transverse slice with a razor blade to provide a uniform flat surface. Slice sections rapidly as described earlier & float them in a Petri dish of water. Treat these like free-hand sections. If commercial Pith is unavailable, find a local plant that has a healthy Pith and use it. If the Pith is still alive, cellular debris may contaminate the sections. Locally, hau (Hibiscus tiliaceus) stems have an execellent Pith! |