Fluorescence Microscope Images

*Longitudinal sections of Cucumis* stems were stained with Aniline Blue and photographed with a fluorescence microscope after excitation with Violet Light. Callose in the Phloem is fluorescent and appears bright under these conditions. It has a yellow/green color. This is partly due to the blue color of the dye. The Sieve Plates are especially fluorescent because they contain a lot of associated Callose and Callose accumulates there upon injury. Lateral Sieve Pores are also fluorescent. Xylem is autofluorescent and appears similar to the Phloem. Xylem can be identified because of its characteristic secondary walls. Plastids appear a red dots due to the fluorescence of Chlorophyll There have been no modifications of the raw scanned images!!!** The times below each photo indicate the time that the shutter was OPEN!
20X Magnification
1 Second	2 Seconds
4 Seconds	8 Seconds
15 Seconds	30 Seconds
60 Seconds
40X Magnification of a Sieve Plate
1 Second	2 Seconds
4 Seconds	8 Seconds
15 Seconds	30 Seconds
60 Seconds	120 Seconds