Monocot Roots
Root Directory
The organization of Monocot roots like Sugarcane is similar to that
found in dicots like Ranunculus. However, tracheary
elements may be absent from the center of the root. |

Transverse Section of Smilax (Cat Briar)
|

Epifluorescence Image of Smilax Root |
  |
Corn
Root: Interface of the Stele & the Cortex-The cells with the largest Diameters
are Vessel Members. Follow the Cortical
Parenchyma from the top of the image to the first cells which have thick,
red-stained walls. These are the Endodermis. |
The Endodermis
usually develops exceedingly thick secondary walls at levels
in the root which are no longer absorbing water. This obscures the Casparian Strips but makes the Endodermis more
obvious.
There are usually more xylem arms in monocots.
The number
of arms is indicated by the following terms.

Number
of Xylem Arms
| Two = Diarch |
| Three = Triarch |
| Four = Tetrarch |
| Five = Pentarch |
| Six = Hexarch |
| More than five = Polyarch |
Monocots are usually Polyarch.
Bundles
of Phloem alternate with the Xylem arms.
The xylem contains many lignified cells in older portions of the root. The phloem stands out because its cells have thin,
unlignified cell walls.

|
Cross-section of Clintonia (above) &
Smilax (below) Roots. Count the number of xylem
arms. Both are Monocots |

|

|
Cross-section
of Smilax Root. The tracheary elements of the Xylem are the cells with the
largest diameters. Count the number of xylem arms. The
Endodermis is the densely stained, lignified layer which surrounds the Stele.
Smilax Stele showing
bundles of Phloem alternating with Xylem Vessel Members.
The cell pattern is distorted by the extreme enlargement of
the Vessel Members. |
Smilax
root at higher magnification. Locate the Xylem and Phloem as well as the
Endodermis, Cortex and Epidermis.
Smilax root showing the Stele, Endodermis, Cortex, Exodermis & Epidermis. The
Exodermis has the same anatomy as the Endodermis
and probably restricts water loss to the outside in older roots.
|

Inner Cortex and Stele of a Pineapple Root Stained with
Toluidine Blue |

Stele from Pineapple Root
lightly Stained with Toluidine Blue |
| Locate the Endodermis
and Xylem! |

Autofluorescence Image of an Orchid Aerial Root: Locate the Velamen and the
Vascular Cylinder. |
 |
 |
Vascular Tisues in the Root of Pili Grass Stained with
Toluidine Blue (Left) & Phloroglucinol (Right). Note the high degree of Lignification. |
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