Bot 311 Study Guide – Photosynthesis – Name ______________________________________

 

The Visible region of the spectrum is also known as PAR which means P_____________________ly A______________e R_______________________ion. It spans the region from ________ to ________ nanometers or the B________e to R___d spectral regions.


Place the Letter for the Best Match inside the Boxes

A] Chlorophyll Absorption

B] Solar Radiation Output

C] Solar Radiation that Penetrates our Atmosphere


When a C_________________l M____________________e absorbs a photon of L___________t, one of its E________________s makes an energy transition to an “E________________ed S______________e”. This represents an increase in P___________________l E_____________y.

During Photosynthesis ____2 is captured and ____2 is produced. It takes L___________t & 1000s of C____________________l molecules to produce 1 molecule of  ____2. Studies have shown that there are 2 R_________________n C______________s located in the T____________s that H________________t L_____________t and produce _____2. These are called PS __ and PS __.  These are linked by E____________n C______________rs. Each PS has a special C_______________l a molecule at its core. These R___________n C__________r C________________s are known as P_____ and P_____ because they strongly a__________b L_____________t at these W__________________s. ______2 fixation occurs in the S___________a of the Chloroplast.

Light Harvesting 1,2,3……..4

Light is a_____________d by a___________a p_____________s and its energy is t____________d to the R____________n C____________r C________s. This e__________s an e____________n in each. These e_____________s are transferred to e_____________n c____________s. The electron from PS  r______________s the e______________n lost by PS ___. An e___________n is taken from W___________r to replace the missing PS ___ e_____________n. This leads to O___________n E________________n. The PS ___ e_____________n is used to R_______________e N________ to N__________ (NADP effectively gains an electron). N__________ is used to Reduce C____________n D__________e in the S_________a of the Chloroplast.

The Oxidation of Water produces an H_______________n ion (Proton). Protons are also transported across the T_______________d during E_______________n T____________________rt. Protons accumulate in the T_________________d L_________________n. This makes one side of the membrane A___________c because it is high in Protons. An A___Synthase in the T________________d Membrane uses the Proton Gradient to produce ___ ___ ___.

OVERALL, The immediate products of Light Harvesting are O___________n, A___P & N__________H. The latter two are used to R_____________e C___________n D___________e in the S____________a.

The A____________a P______________s & Light Harvesting Complexes are conceptually organized like a F___________l with a S____________e at the b_____________m.


Place the Letter for the Best Match inside the Boxes

A] Chlorophyll A

B] Chlorophyll B

C] Carotenoids

D] Reaction Center

 

 

The A_______________a P______________s in Land Plants and Green Algae are Chlorophyll ___ & ___, plus various C______________s. C________________s absorb in the b__________e-g__________n region of the spectrum and b____________n the range of w_________________s that can be harvested for Photosynthesis. Other organisms, like Red Algae (Rhodophyta) have different A______________a P_______________s which we will study later.


Place the Letter for the Best Match inside the Boxes

A] Chlorophyll A

B] Chlorophyll B

C] Carotenoids

D] Bacteriochlorophyll

 

 

Carbon Fixation – C3 Photosynthesis

There are three basic events  which characterize _____2 F_________________ion.  These are C_________________n, R_______________n & R___________________n. The first step involves the addition of _____2 to a 5-C___________n Acceptor. This leads to the production of two 3-C___________n molecules. The next step involves the R____________n of the 3-C_____________n molecule into a C__________________te. The final step is a complex series of reactions, which R___________________s the 5-C________________n A________________r R_______P.

The enzyme that adds CO2 to RUBP is called R____________________O (Ribulose Bisphosphate Carboxylase/Oxygenase). It has a dual function. It can Carboxylate RUBP (Photosynthesis) or it can O______________e it (P__________________n). This latter results in the loss of ______2! Both of these reactions involve the same active site, and the substrates directly C_________________e with one another. Under typical ambient conditions the ratio between CO2 fixation & Oxygenation is ___:1. Photorespiration reduces the efficiency of photosynthesis by as much as ______%. Concentrations of _____2 & ______2, plus the Leaf T________________e regulate the balance between P_______________s & P____________________n. C____________n D____________e concentrations are always much lower than O___________n levels inside leaves and in the atmosphere. L__________f T________________e rises during photosynthesis, especially under dry, sunny conditions. This favors P__________________n over P_______________________s.

C-4 Photosynthesis

Some plants have Carboxylating enzymes that have a higher affinity for ___2, especially at l___w c______________s & h_______h t___________________s. These use 3-Carbon acceptors like PEP rather than RUBP. Carboxylation produces a ___-Carbon acid like M____________te. The Vascular Bundles of C4 leaves have large B___________e S___________h cells that contain C____________s. 1-3 layers of P___________________c M_________________l cells surround these. This resembles a wreath and this has been called K_______z Anatomy. This M________________s & D_____________s.

Place the Letter for the Best Match inside the Boxes

A] Bundle Sheath

B] Photosynthetic Mesophyll

 

 

There are two types of C___________________s in the M_________________l &  B_____________e S_____________h cells of C4 Leaves.

Chloroplasts with G___________a are observed in M______________l Cells. Chloroplasts without Grana are found in the B______________e S____________h cells. S_______________h accumulates in the Bundle Sheath Chloroplasts during active periods of P___________________s. It is transported to the Phloem during the night.

Place the Letter for the Best Match inside the Boxes

A] Mesophyll Chloroplast

 

B] Bundle Sheath Chloroplast

 

Carbon Path in C4 Psyn:  _________2 crosses the Cell Wall and Plasmalemma of a M______________l C___________l. It is fixed by ______ Carboxylase to form a 4-C__________n Acid like Malate. This occurs in the C__________________m. The 4-Carbon Acid is t_________________d via P____________________a to a B_____________e S_______________h C________l where ______2 is released and fixed by R____________O using the C____Cycle. PEP is reformed and is t________________d back to the M________________l Cell where it can accept another _________2.

Place the Letter for the Best Match inside the Boxes

A] PEP (used twice)

B] PEP Carboxylase

C] CO2 (used twice)

D] Cell Wall

E] Plasmalemma

F] Plasmodesmata

G] Malate

H] RUBISCO

I] 3 Carbon Unit

Label the Bundle Sheath Cell & the Mesophyll Cell

 

 

C4 plants photosynthesize better than C3 plants under d___y h____t conditions. The greater a__________y of PEP Carboxylase for ____2 means that the enzyme is s_____________d at low ______2 levels. Consequently, stomata may be c________________d for longer periods of time with C___ plants versus C____ plants. This helps to conserve w________r. PEP Carboxylase is not inhibited by ____2 and is incapable of P_________________n. This C4 process  c___________________s ______2 in the vicinity of R_____________O in the B______________e S______________h C_________s where the C_______________s do not produce _____2 because they lack G________a. This favors P__________________s over P______________________n in the Bundle Sheath Cells.

 

Place the Letter for the Best Match inside the Boxes

 

A] C3 Plant

 

B] C4 Plant

 

 

CAM Photosynthesis: Crassulacean Acid Metabolism (CAM) is another way in which plants concentrate ____2. Stomatal opening is regulated so that they O______n at N__________t when W__________r d________d is low. CAM plants can show an _____% reduction in water loss compared to C3 plants. CAM plants  fix CO2 via P__P C______________e. They store M_______e in their V____________s. This is transported to C____________s during the d___y where D__________________n occurs. The released ____2 is fixed by R_______________O. Since the stomata are closed during the day neither _____2 or W___________r can escape. Virtually all of the ______2 is fixed and P___________________n is negligible.

 


Place the Letter for the Best Match inside the Boxes A] PEP (used twice)  B] PEP Carboxylase   C] CO2 (used twice)   D] Day   E] Night   F] Vacuole  G] Malate (used 4 times)  H] RUBISCO     I] C3 [Calvin] Cycle    J] Open Stomate   K] Closed Stomate     L] Vacuole (used twice)

 

Leaves are Green because they either r_____________________t or a__________________b other w__________________hs of l______________t.

Some Epidermal cells are able to f____________s light on p_________________ic M___________________l c____________s.

The number of P_____________e layers is related to the amount of light reaching the leaves. Many layers are present in b___________t l____________t environments while one or two are found in d___m light. P_____________e Cells have s___________l i______________r s______________s. However, their shape provides a lot of s_____________e area for g___s e_______________e without e_______________e a___r s_____________s. Consequently, they have the best Shape & Organization for photosynthetic efficiency. Multiple P_________________e layers are found in plants which inhabit d_______y environments.

The Sieve Effect is due to the fact that c_______________l is contained in C_____________________s rather than being dispersed uniformly throughout the cell. Light Channeling occurs because C_______________s are distributed in the o____________________t C___________________m. This leaves a t_____________________t central region occupied by the V____________________e. Light is channeled through without being absorbed. Light Channeling also occurs in the A_________________t . Light Scattering also occurs in the A________________t. This diffuses the light &  i________________s the path of photons within the leaf This i_______________s the probability of it’s a_________________n. Photon paths within leaves can be increased by a factor of F__________R! The S_____________y M_________________l has large I_________________r S______________s and irregularly o_______________d cells. Both of these factors i_______________e the light path within leaves. This makes light a______________n more likely!

Leaves have many adaptations which can i__________________t the a_________________n of e________________s light. These are primarily found in the E_______________s but may also include other tissues. Some of these r_____________t light while others may s__________r or a_____________b light. T_________________s tend to r_________________t light, as may a thick W__________y layer or C__________________e. Irregularly E__________________d C__________s may also be reflective.

 

Place the Letter for the Best Match in the Boxes     A] Light Channeling   B] Vacuole   C] Chloroplasts

 

Kukui Trees are easily identified due to their highly r________________e leaves. This is due the presence of dense, multicellular T______________s. The reflection of ohi'a lehua leaves is due to a dense layer  of T__________________s. These typically occur on the l______________r surface of the leaf but can be abundant on the u_______________r surface as well. The Leaves of Ohi'a lehua have a multilayered H__________________s which may also decrease the amount of light reaching the P_____________________ic M___________________l.

 

Place the Letter for the Best Match in the Boxes    

A] High Light

 

B] Low Light

The disposition of the C___________________s can control the amount of L______________t absorbed by leaves. Chloroplasts are a_______________d  and d_____________d p________________________r to the light when light levels are l_____w. This achieves m____________________l a______________________n. The converse is true under h___________h l_____________t conditions. Chloroplast orientation can change the amount of light absorbed by ________%.

Chloroplast movement also occurs in A_________e. Species of Mougeotia have flat ribbon-like chloroplasts These are p________________r to the L_______________t at l_____w irradiances. They have a p______________l orientation at h_____________ irradiances.

 

Place the Letter for the Best Match in the Boxes    

A] High Light

 

B] Low Light

 

C] Chloroplast

Some plants can change the o________________________n of their leaves t_______________s the Sun. This is called Solar Tracking. In some cases this adjustment is c___________________s throughout the day so the leaf blades are always p______________________r to the sun's rays. In many cases the leaves of solar-tracking plants have a P___________________s. This is a swollen part of the P_______________e and may occur where it joins the stem, the leaf blade or BOTH. The P_____________s contains M__________r C________s which control the disposition of the petiole and consequently, the blade. Solar Tracking may be most beneficial in the early m_____________________g and late a______________n when the w___________r b_________________e of the leaf is f_________________e for P___________________s but the amount of available l_____________t is low. This is similar to what occurs when the V________s F_____________p snaps shut. This is controlled by changes in T_____________r P__________________e. The shape of G____________d C____________s is regulated in a similar manner.

Shady zones may only receive _____% of the PAR received in open environments. Shade Leaves have more C_______________l per R_____________n C______________r, and more C__________________l b which is an A____________a  P_________________t that expands the spectral region over which L_______________t can be a_______________________d.  Plants grown at low light have greatly reduced R_____________________n rates, which allows them to reach their Light C_______________n Point (LCP) at l___w irradiance. The LCP is the irradiance at which the _______2 fixed by Photosynthesis is equal to the ______2 lost by Respiration. The Light S______________n Point is reached when further increases in i_________________e fail to i_______________e _____2 fixation. As Shade leaves are S___________________d at lower i___________________s than sun leaves. Shade leaves can not s_________________e at high irradiances!

 

Place the Letter for the Best Match in the Boxes    

 

A] Sun Leaf

 

B] Shade Leaf

C] Compensation Point

D] Saturation

 

 

Evaporative or Latent heat loss occurs when w___________r evaporates from the c________l w___________s of leaf cells. This is due to the latent heat of evaporation. T______________________n is the major source of evaporative heat loss. Sensible or Perceptible heat loss occurs when captured s_____________t wavelength light energy is r_______________d as l_________g wavelength irradiation. Plants that primarily use sensible heat loss to shed excess energy must keep their s_________________a closed. These plants must endure very high leaf temperatures in order to survive. This obviously restricts CO2 uptake and leads to s________w growth.