Biology 270L
Cell and Molecular Biology Lab
General Information
- Instructor: Dr. Maq Alam (Snyder 111, 956-6945)
- Laboratory:
Laboratory Topics
- Laboratory orientation and videos
- Determination of live and dead eukaryotic and prokaryotic cells by
fluorescence microscopy
- Bacterial chemotaxis
- Assembly of microtubules in flagella of Chlamydomonas
- Immunofluorescence microscopy: visualization of organelles in
Paramecium by monoclonal antibody
- Principles of enzyme catalysis
- Isolation of genomic DNA, agarose gell electrophoresis
- Restriction enzyme analysis of genomic and plasmid DNA
- Polymerase Chain Reaction
- Protein fingerprinting I: preparation of samples, assembly and
pouring of acrylamide gels
- Protein fingerprinting II: running and analysis of SDS-PAGE
- Introduction to computer analysis of the 3-D structure of DNA and
RNA
- Introduction to computer analysis of the 3-D structure of amino
acids and proteins
Laboratory Notebook
You will be required to keep a notebook for Biology 270L. We recommend
getting a bound or spiral notebook with lined or quadrille pages
(100-200). A bound notebook will ensure that important pges of data are
not misplaced, and quadrille pages are very convenient when you need to
plot data.
You should always bring your notebook with you to lab.
Lab Manual
You should bring $5.00 to the first meeting of your lab section to
purchase the Biology 270L lab manual.
Disclaimer: This information has been obtained from the syllabus
for the Spring 1996 class offering and is only partial information
about the course. It is not an authorized syllabus and does not
offer any guarantee that the course was taught according to this outline
then or will be taught this way in the future. It is only intended for
general planning.