Leaf Anatomy


Close-up of leaf, showing conceptacles.


Conceptacle x.s., showing sterile "hairs", 25x.

Nonreproductive conceptacles are visible on the leaf surface as dark spots. A cross-section through one shows its structure. In a fertile conceptacle, the sterile hairs would be accompanied by antheridia or oogonia. 


Leaf x.s., 25x.

A cross-section through the entire leaf shows differentiation into epidermis, cortex, and medulla. The epidermis may be differentiated into a meristoderm and cuticle.


(above) Leaf epidermis and outer cortex, 25x. Polarized.
(below) Leaf epidermis and outer cortex, 25x. Non-polarized.


Leaf surface, 63x. Partly polarized.


A cross-section through the leaf surface shows both a pigmented epidermal layer and an unpigmented inner cortical layer. Note the large size of the cortical cells as compared to the epidermal cells. A partly polarized view of the surface of the leaf also shows the pigmented epidermal cells.



(above) Leaf cortex, 160 x, unpolarized.
(below) Leaf cortex, 160x, polarized.


Polarized and upolarized cross-sections through the cortex show the thick cell walls.



Leaf cortex and epidermis, stained with aniline blue. 100x.

(above)Medulla and cortex, stained with aniline blue, polarized. 100x.
(below) Medullary cells, 160x.


A stained cross-section through the cortex shows the thick cell walls more clearly. Note the absence of staining within the cells. A medullary cross-section shows what may be the uptake of stain by the conductive cells (this image is polarized, so the cell walls are birefringent). Non-stained images of the medulla show the more densely pigmented and smaller cells, with thicker cell walls as compared to the epidermis. Because of this pigmentation, it is difficult to tell whether there is a great deal of stain uptake in these cells.


(above) Transverse section through leaf, polarized and stained with aniline blue. Long axis is oriented vertically. 25x.
(below) Transverse section through medulla, axis oriented horizontally. 100x.

A transverse section through the leaf shows the elongated medullary cells. A closer view shows the presence of what look like sieve plates between adjoining cells.